Effects of ursolic acid on the structural and morphological behaviours of dipalmitoyl lecithin vesicles

TitleEffects of ursolic acid on the structural and morphological behaviours of dipalmitoyl lecithin vesicles
Publication TypeJournal Article
Year of Publication2015
AuthorsLorincz, A, Mihály, J, Németh, C, Wacha, A, Bóta, A
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1848
Issue5
Pagination1092 - 1098
Date Published2015
ISBN Number0005-2736
KeywordsDPPC, Freeze-fractured transmission electron-microscopy (FF-TEM), Kratky camera, Non-bilayer lipid structure, Small- and wide-angle X-ray scattering (SWAXS), Ursolic acid
Abstract

Effects of ursolic acid on the structural and morphological characteristics of dipalmitoyl lecithin(DPPC)-water system was studied by using differential scanning calorimetry (DSC), small- and wide-angle X-ray scattering (SWAXS), freeze-fracture method combined with transmission electron-microscopy (FF-TEM) and infrared spectroscopy (FT-IR). The surface of the uncorrelated lipid system is rippled or grained and a huge number of small, presumably unilamellar vesicles are present if the UA/DPPC molar ratio is 0.1 mol/mol or higher. Besides the destroyed layer packing of regular multilamellar vesicles, non-bilayer (e.g. cubic or hexagonal) local structures are evidenced by SAXS and FF-TEM methods. The ability of UA to induce non-bilayer structures in hydrated DPPC system originates from the actual geometry form of associated lipid and UA molecules as concluded from the FT-IR measurements and theoretical calculations. Beside numerous beneficial e.g. chemopreventive and chemotherapeutic effect of ursolic acid against cancer, their impact to modify the lipid bilayers can be utilized in liposomal formulations.

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84923070939&partnerID=40&md5=1e9ee3841a9273c0536a87417fc09bf5
Short TitleBiochimica et Biophysica Acta (BBA) - Biomembranes