Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles

TitleNanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles
Publication TypeJournal Article
Year of Publication2020
AuthorsDeak, R, Mihály, J, Szigyártó, ICs., Beke-Somfai, T, Turiák, L, Drahos, L, Wacha, A, Bóta, A, Varga, Z
JournalMaterials Science and Engineering: C
Pagination110428
ISSN0928-4931
Abstract

A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.

URLhttp://www.sciencedirect.com/science/article/pii/S0928493119315450
DOI10.1016/j.msec.2019.110428