Direct immobilization of manganese chelates on silica nanospheres for {MRI} applications

TitleDirect immobilization of manganese chelates on silica nanospheres for {MRI} applications
Publication TypeJournal Article
Year of Publication2017
AuthorsPálmai, M, Pethő, A, Nagy, LNaszályi, Klébert, S, May, Z, Mihály, J, Wacha, A, Jemnitz, K, Veres, Z, Horváth, I, Szigeti, K, Máthé, D, Varga, Z
JournalJournal of Colloid and Interface Science
Pagination298 - 305
KeywordsNanoparticulate {MRI} contrast agent

Abstract The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T1) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn2+. After comprehensive characterization, the {MRI} properties of functionalized {MSNSs} were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro {MRI} investigations, which was also evidenced by significant contrast enhancement on T1-weighted {MR} images in vivo. Moreover, in vitro cytotoxicity assay of functionalized {MSNSs} on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn2+-chelating {MSNSs} are appropriate candidates for liver-specific T1-weighted {MRI} {CAs} with high relaxivities (r1 = 7.18 mM−1 s−1).