Preparation and 68Ga-radiolabeling of porous zirconia nanoparticle platform for PET/CT-imaging guided drug delivery

TitlePreparation and 68Ga-radiolabeling of porous zirconia nanoparticle platform for PET/CT-imaging guided drug delivery
Publication TypeJournal Article
Year of Publication2017
AuthorsPolyak, A, Nagy, LNaszályi, Mihály, J, Görres, S, Wittneben, A, Leiter, I, Bankstahl, JP, Sajti, L, Kellermayer, MSZ, Zrínyi, M, Ross, TL
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume137
Pagination146 - 150
ISSN0731-7085
KeywordsImaging guided therapy
Abstract

Abstract This paper describes the preparation of gallium-68 (68Ga) isotope labeled porous zirconia (ZrO2) nanoparticle (NP) platform of nearly 100 nm diameter and its first pharmacokinetic and biodistribution evaluation accomplished with a microPET/CT (μPet/CT) imaging system. Objectives of the investigations were to provide a nanoparticle platform which can be suitable for specific delivery of various therapeutic drugs using surface attached specific molecules as triggering agents, and at the same time, suitable for positron emission tomography (PET) tracing of the prospective drug delivery process. Radiolabeling was accomplished using {DOTA} bifunctional chelator. {DOTA} was successfully adsorbed onto the surface of nanoparticles, while the 68Ga-radiolabeling method proved to be simple and effective. In the course of biodistribution studies, the 68Ga-labeled DOTA-ZrNPs showed proper radiolabeling stability in their original suspension and in blood serum. μPet/CT imaging studies confirmed a RES-biodistribution profile indicating stable nano-sized labeled particles in vivo. Results proved that the new method offers the opportunity to examine further specifically targeted and drug payload carrier variants of zirconia {NP} systems using PET/CT imaging.

URL//www.sciencedirect.com/science/article/pii/S0731708516310147
DOI10.1016/j.jpba.2017.01.028