|Title||Silica@zirconia@poly(malic acid) nanoparticles: Promising nanocarriers for theranostic applications|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Nagy, LNaszályi, Polyak, A, Mihály, J, Szécsényi, Á, Szigyártó, ICs., Czégény, Z, Jakab, E, Németh, P, Magda, B, Szabó, P, Veres, Z, Jemnitz, K, Bertóti, I, Jóba, RP, Trencsényi, G, Balogh, L, Bóta, A|
|Journal||Journal of Materials Chemistry B|
|Keywords||Beta-cyclodextrin, Concentration-dependent, Core shell structure, Diagnosis, Lyophilized powders, Nanoparticles, Phosphate buffers, Proof of concept, Stable suspensions, Suspensions (fluids), Targeted delivery, Zirconia|
Silicaatzirconiaatpoly(malic acid) nanocarriers of 110 nm mean diameter were designed, synthesized and characterized for the targeted delivery of diagnostic and therapeutic 99mTc to folate-overexpressing tumors. An important achievement was that a multifunctional l-(-)-malic-acid-based copolymer was formed in situ at the surface of the inorganic cores in a single synthetic step incorporating l-(-)-malic acid, β-cyclodextrin rings, folic acid moieties, and polyethylene glycol chains. Morphological and in-depth structural analysis of the particles proved their core@shell structure. Stability experiments in aqueous media evidenced that stable suspensions can be obtained from the lyophilized powder in 10 mM phosphate buffer at pH 7.4. During 14-day degradation experiments, the nanoparticles were found to be slowly dissolving (including inorganic core) in saline and also in total cell medium. An in vitro toxicity assay on hepatocytes showed a concentration-dependent decrease of cell viability down to 63 ± 1% at the highest applied concentration (0.5 mg ml-1). Proof of concept experiments of technetium-99m radiolabelling and in vivo labelling stability are presented. © The Royal Society of Chemistry 2016.