Serum albumin coating of demineralized bone matrix results in stronger new bone formation

TitleSerum albumin coating of demineralized bone matrix results in stronger new bone formation
Publication TypeJournal Article
Year of Publication2016
AuthorsHorváthy, DB, Vácz, G, Szabó, T, Szigyártó, ICs., Torõ, I, Vámos, B, Hornyák, I, Renner, K, Klára, T, Szabó, BT, Dobõ-Nagy, C, Doros, A, Lacza, Z
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Pagination126 - 132
Date Published2016
ISBN Number15524973 (ISSN)
Keywordsanimal cell, animal experiment, animal model, Article, biomechanics, Body fluids, bone, bone defect, bone densitometry, bone density, Bone formation, bone mass, bone matrix, Bone replacement, bone transplantation, Breaking force, calvaria defect, cell adhesion, cell function, Cells, Coatings, Computerized tomography, Control groups, controlled study, Cytology, DBM, Defects, Demineralized bone matrix, ex vivo study, force, in vitro study, in vivo study, male, micro-computed tomography, Nonhealing defects, nonhuman, ossification, postoperative period, rat, rigidity, Serum Albumin, skull defect, stem cell, stem cells, tissue graft

Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMS at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells. © 2015 Wiley Periodicals, Inc.

Short TitleJ. Biomed. Mater. Res. Part B Appl. Biomater.