Determination of absolute configuration of ketamine enantiomers by HPLC- CD-UV technique

TitleDetermination of absolute configuration of ketamine enantiomers by HPLC- CD-UV technique
Publication TypeJournal Article
Year of Publication1999
AuthorsGergely, A, Zsila, F, Horváth, P, Szász, G
JournalChirality
Volume11
Issue10
Pagination741 - 744
Date Published1999
KeywordsAbsolute configuration, Chiroptical detector, HPLC, Ketamine enantiomers, Octant rule
Abstract

Recognizing that the stereochemical structure of NMDA receptor antagonist ketamine provides valuable data about the relationship between its conformation and absolute configuration by CD-UV analysis, a method for the identification of ketamine enantiomers is proposed which avoids the need for authentic samples of the enantiomers. The ketamine enantiomers were separated by HPLC using Chiralcel OJ stationary phase. The in situ registration of CD and UV spectra, together with the application of the octant rule for cyclohexanone derivatives, makes possible the direct assignment of the eluted ketamine enantiomers.

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