Binding of the pepper alkaloid piperine to bovine β-lactoglobulin: Circular dichroism spectroscopy and molecular modeling study

TitleBinding of the pepper alkaloid piperine to bovine β-lactoglobulin: Circular dichroism spectroscopy and molecular modeling study
Publication TypeJournal Article
Year of Publication2005
AuthorsZsila, F, Hazai, E, Sawyer, L
JournalJournal of Agricultural and Food Chemistry
Volume53
Pagination10179-10185
Abstract

{The pepper alkaloid piperine is a nontoxic, natural dietary compound with a broad range of physiological activity. The present work is the first demonstration of its interaction with a mammalian protein. Circular dichroism (CD) spectroscopy was used to reveal and analyze the binding of piperine to a lipocalin protein. Induced CD spectra measured in pH 7.7 phosphate buffer at 37°C demonstrated reversible, non-covalent association of piperine with bovine β-lactoglobulin (BLG), the major whey protein in milk. The binding parameters (Ka ≈ 8 × 104 M-1

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-30544433324&partnerID=40&md5=7000d9382db7a91494e08da756033c0f
DOI10.1021/jf051944g
PubMed ID16366712